ABSTRACT
The evaluation of microbiological quality of tank water at the Institute of Management and Technology campus III Enugu was carried out by using ten samples of water obtained from ten different tank waters. For viable plate count for the presence of pathogenic organisms and organisms indicating organic matter pollution, plating technique of pour plates were used, also presence of coliform groups and Escherichia coli were examined using the Presumptive, Confirmatory and Completed coliform test techniques. The media used include Nutrient agar, Brilliant green bile agar, Eosin Methylene Blue agar (EMB), MacConkey broth and Brilliant green bile broth. The total viable plate count gave 31 X 10-3 cfu/ml for pathogenic organisms and 21 X 10-3 cfu/ml for organisms indicating organic matter pollution, whereas the presumptive test showed the presence of coliform groups in seven (7) samples of water out of ten. The confirmatory test showed the presence of colform in five (5) samples of water whereas the presence of Escherichia coli was shown in three (3) samples. The completed test result revealed a highest coliform count of 2 cfu in one sample of water while 1cfu was
obtained in three (3) water samples for Escherichia coli. The result indicated the presence of coliform and Escherichia coli but from the
deduction, the microbial load was minimal in all the tests. Therefore, the tank water at campus III of IMT is not fit for drinking though it
can be used for other domestic purposes.
TABLE OF CONTENTS
Title page i
Certification ii
Dedication iii
Acknowledgement iv
Table of contents v
List of Tables viii
Abstract ix
CHAPTER ONE
INTRODUCTION
Background of Study 1
Statement of the Problem 2
Objective of the Study 3
Hypothesis 3
Significance of the study 3
Limitations of the study 4
CHAPTER TWO
LITERATURE REVIEW 5
Sources of Tank Water 6
Drinking Water 8
Drinking Water Standard 8
Tank water Contamination 10
Tank Water purification/Treatment 12
Water pollution Indicator 15
CHAPTER THREE
MATERIALS AND METHODS
Materials (see appendix) 18
Methods 18
3.2.1 Sterilization of Materials 18
Collections of Water Samples 18
Preparation of Culture Media 19
Dilution of Water Samples 19
Examination of Water Samples
Total Viable Plate Counts 20
Bacteriological Examination 21
CHAPTER FOUR
RESULT 25
Total Viable Plate Counts 25
Coliform Test
Presumptive Test 26
Confirmatory Test 27
Completed Test 29
CHAPTER FIVE
DISCUSSION, CONCLUSION AND RECOMMENDATION
Discussion 30
Conclusion 33
Recommendation 34
REFERENCES 35
APPENDIX 37
LIST OF TABLES
Table 1:
Total Viable Plates Count for pathogenic organisms and organisms for organic matter pollution present in the ten samples of water 25
Table 2: Presumptive test for the number of tubes positive for coliform organisms 26
Table 3: Confirmatory test for coliform organisms and Escherichia coli present in the water samples and their most probable number (mpn). 27
Table 4: Completed test for coliform organisms and Escherichia coli. 29
